Coding
RESET

Part:BBa_K1433012:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)


Terminator-RFP-RBS-attL-Promotor-attR-RBS-GFP-Terminator-Promotor-Xis-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 920
    Illegal NheI site found at 943
    Illegal NheI site found at 3087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 873
    Illegal NgoMIV site found at 3121
    Illegal AgeI site found at 2861
    Illegal AgeI site found at 3889
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 893
    Illegal BsaI.rc site found at 976
    Illegal BsaI.rc site found at 1669
    Illegal SapI site found at 2843


Design Notes

no


Source

Derive from 2014 Distribution Kit.

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.